P5431

Pectolyase from Aspergillus japonicus

aseptically filled, lyophilized powder, ≥2 units/mg solid

• EC Number: 3.2.1.15 (BRENDA, IUBMB)
• MDL number: MFCD00131813
• UNSPSC Code: 12352204

Properties

• sterility: aseptically filled
• form: lyophilized powder
• specific activity: ≥2 units/mg solid
• storage temp.: 2-8°C

Description

Application

Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Other Notes

Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor.

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Unit Definition

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 5.5 at 25 °C.

Physical form

Lyophilized powder containing lactose

Preparation Note

Prepared from P 3026

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves