U0631

Urea

BioXtra, pH 7.5-9.5 (20 °C, 5 M in H₂O)

• Synonym(s): Carbamide, Carbonyldiamide
• Linear Formula: NH₂CONH₂
• CAS Number: 57-13-6
• Molecular Weight: 60.06
• Beilstein: 635724
• EC Number: 200-315-5
• MDL number: MFCD00008022
• UNSPSC Code: 12352111
• PubChem Substance ID: 24900617
• NACRES: NA.31
• Assay: 98.5-101.5%
• Application(s): diagnostic assay manufacturing

Properties

• product line: BioXtra
• Quality Level: 200
• Assay: 98.5-101.5%
• form: powder, crystals or pellets
• impurities: ≤0.005% Phosphorus (P); ≤0.1% Insoluble matter
• ign. residue: ≤0.1%
• pH: 7.5-9.5 (20 °C, 5 M in H₂O)
• mp: 132-135 °C (lit.)
• solubility: H₂O: 5 M at 20 °C, clear, colorless
• density: 1.335 g/mL at 25 °C (lit.)
• anion traces: chloride (Cl^-): ≤0.05%; sulfate (SO₄^2-): ≤0.05%
• cation traces: Al: ≤0.0005%; Ca: ≤0.001%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Mg: ≤0.0005%; NH₄⁺: ≤0.05%; Na: ≤0.005%; Pb: ≤0.001%; Zn: ≤0.0005%
• absorption: ≤0.06 at 260 nm at 5 M; ≤0.06 at 280 nm at 5 M
• application(s): diagnostic assay manufacturing
• SMILES string: NC(N)=O
• InChI: 1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
• InChI key: XSQUKJJJFZCRTK-UHFFFAOYSA-N
• Gene Information: human ... CA1(759), CA2(760) ; rat ... Ppm1a(24666)

Description

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves