J774A.1 Cell Line from mouse

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 91051511, mouse blood, Not specified

• Sinónimos: J774 A1 Cells, J774.A1 Cells, J774A1 Cells
• Código UNSPSC: 41106514

Propiedades

• origen biológico: mouse blood
• descripción: Mouse BALB/c monocyte macrophage
• envase: tube of 5 μg 91051511-DNA-5UG; pkg of vial of cells 91051511-1VL
• modo de crecimiento: Semi-adherent
• cariotipo: Not specified
• morfología: Not specified
• productos: Interleukin 1 (IL-1), lysozyme
• receptores: Immunoglobulin, complement
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse BALB/c monocyte macrophage

Descripción línea celular

Derived from a tumour in a female BALB/c mouse. Cell line produces IL-5 and lysozyme.

Aplicación

J774A.1 cell line has been used to test the cytotoxicity of [1,2,3]triazolo[1,5-a]pyridinium salts (having leishmanicidal activity) from triazolopyridines. It has also been used to determine the antitrypanosomatid activity of flavonoid glycosides isolated from Delphinium sps.

Metabolic studies

Medio de cultivo

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Cells are subcultured by harvesting attached and suspension cells. Remove majority of medium and centrifuge to harvest cells growing in suspension. Scrape adherent cells remaining in the flask. Resuspend cell pellet from centrifugation and combine with scraped cells. Seed into new flask at 1:3 to 1:4 split ratio i.e. seeding at 1-3 x 10,000 cells/cm² Incubate at 37°C; 5% CO₂. Please Note :- On receipt growing order cells may be in suspension. The cells and media should be removed from the flask and spun down to form a pellet. This should then be re-suspended in fresh media and a cell count performed. The cells should then be reseeded at 2-4 x 10E4/cm and should begin to show signs of re-attachment within 48 hours.

Otras notas

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