Skip to Content
Merck
Sign Into View Organizational & Contract Pricing

About This Item

Empirical Formula (Hill Notation):
C37H27N3Na2O9S3
CAS Number:
28983-56-4
Molecular Weight:
799.80
MDL number:
MFCD00058509
UNSPSC Code:
41116130
EC Index Number:
249-352-9
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Quality Level

400

form

solid

potency

>5000 mg/kg LD50, oral (Rat)

composition

Dye, ≥60% spectrophotometric assay

pH

5.0 (20 °C, 10 g/L in H2O)

solubility

70 g/L

bulk density

670 kg/m3

εmax

675-1125 at 595-605 nm in 5 mM HCl at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

SMILES string

[Na+].[Na+].[S](=O)(=O)([O-])c1c(cccc1)Nc2ccc(cc2)C(=C5C=CC(=Nc6c(cccc6)[S](=O)(=O)[O-])C=C5)c3ccc(cc3)Nc4c(cccc4)[S](=O)(=O)[O-]

InChI

1S/C37H29N3O9S3.2Na/c41-50(42,43)34-10-4-1-7-31(34)38-28-19-13-25(14-20-28)37(26-15-21-29(22-16-26)39-32-8-2-5-11-35(32)51(44,45)46)27-17-23-30(24-18-27)40-33-9-3-6-12-36(33)52(47,48)49;;/h1-24,38-39H,(H,41,42,43)(H,44,45,46)(H,47,48,49);;/q;2*+1/p-3

InChI key

TUHAIJABPUJAEY-UHFFFAOYSA-K

Looking for similar products? Visit Product Comparison Guide

General description

Methyl blue (C.I. 42780), is a dry dye for the preparation of a color staining solution used for the polychrome staining of histological tissue sections of human origin. The dye is a CE certified IVD product and has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 60 %
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (Hydrochloric acid 0.005 mol/l): 595 - 605 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.01 g/l; hydrochloric acid 0.005 mol/l): 675 - 1125
TLC-Test: passes test
Loss on drying (110 °C): ≤ 7 %
Suitability for microscopy: passes test

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point(C)

> 300 °C - Pensky-Martens closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service