1.16316

Methyl blue (C.I. 42780)

for microscopy

• Synonym(s): Methyl blue (C.I. 42780), Methyl blue for microscopy and microbiology
• Empirical Formula (Hill Notation): C₃₇H₂₇N₃Na₂O₉S₃
• CAS Number: 28983-56-4
• Molecular Weight: 799.80
• MDL number: MFCD00058509
• UNSPSC Code: 41116130
• EC Index Number: 249-352-9

Properties

• Quality Level: 400
• form: solid
• potency: >5000 mg/kg LD₅₀, oral (Rat)
• composition: Dye, ≥60% spectrophotometric assay
• pH: 5.0 (20 °C, 10 g/L in H₂O)
• solubility: 70 g/L
• bulk density: 670 kg/m³
• εmax: 675-1125 at 595-605 nm in 5 mM HCl at 0.01 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C

Description

General description

Methyl blue (C.I. 42780), is a dry dye for the preparation of a color staining solution used for the polychrome staining of histological tissue sections of human origin. The dye is a CE certified IVD product and has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 60 %
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (Hydrochloric acid 0.005 mol/l): 595 - 605 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.01 g/l; hydrochloric acid 0.005 mol/l): 675 - 1125
TLC-Test: passes test
Loss on drying (110 °C): ≤ 7 %
Suitability for microscopy: passes test

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 2
• Flash Point(C): > 300 °C - Pensky-Martens closed cup