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Key Documents

11034731001

Roche

Primer "random"

lyophilized, sufficient for ≤400 reactions, pkg of 2 mg (Primer, Random pd(N)6 Potassium Salt)

Synonym(s):

primer

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About This Item

UNSPSC Code:
41106300

form

lyophilized

Quality Level

usage

sufficient for ≤400 reactions

packaging

pkg of 2 mg (Primer, Random pd(N)6 Potassium Salt)

manufacturer/tradename

Roche

storage temp.

−20°C

General description

p(dN)6

Application

Primer "random" has been used:
  • in a reverse transcription-polymerase chain reaction (RT-PCR) and quantitative PCR (qPCR) to retro-transcribe RNA
  • to reverse-transcribe RNA samples to cDNA
  • in digital droplet PCR (ddPCR) to synthesis cDNA from RNA

Primer for cDNA synthesis

Features and Benefits

  • Offers a consistent representation of all RNA sequences in mRNA
  • Can prime cDNA transcription from RNAs lacking a poly(A) tail
  • Short cDNA transcripts can overcome the challenges posed by RNA secondary structures
  • The average length of cDNAs formed can be controlled by adjusting the ratio of random primers to RNA in the reverse transcription (RT) reaction

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash


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