D7008

Dihydroethidium

≥95% purity, powder

• Synonym(s): 2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, 3,8-Diamino-5,6-dihydro-5-ethyl-6-phenylphenanthridine, Hydroethidine
• Empirical Formula (Hill Notation): C₂₁H₂₁N₃
• CAS Number: 104821-25-2
• Molecular Weight: 315.41
• Beilstein: 5107482
• MDL number: MFCD00077335
• UNSPSC Code: 12171500
• PubChem Substance ID: 24894069
• NACRES: NA.47

Properties

• Product Name: Dihydroethidium, ≥95%
• Quality Level: 100
• Assay: ≥95%
• form: powder
• technique(s): titration: suitable
• color: pink to very dark red
• solubility: chloroform: 20 mg/mL
• fluorescence: λ_ex 370 nm; λ_em 420 nm (for cytoplasm of living cells); λ_ex 535 nm; λ_em 610 nm (for chromatin of living cells)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: −20°C

Description

Application

Dihydroethidium has been used as a superoxide probe in bovine pulmonary artery endothelial cell (BPAEC).[1] It has been used to check the in situ generation of ROS (reactive oxygen species).[2][3]

Redox indicator. Blue fluorescence until oxidized to ethidium.

Packaging

Packaged under Argon.

Linkage

B-ring reduced analog of ethidium

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves