00379

Atto 495 NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

• MDL number: MFCD06798157
• UNSPSC Code: 12352108
• PubChem Substance ID: 329747054
• NACRES: NA.32

Properties

• product line: BioReagent
• Quality Level: 100
• Assay: ≥90% (HPLC); ≥90% (degree of coupling)
• manufacturer/tradename: ATTO-TEC GmbH
• solubility: DMF: soluble; DMSO: soluble; acetonitrile: soluble
• λ: in acetonitrile (with 0.1% acetic acid)
• UV absorption: λ: 496-502 nm A_max
• suitability: suitable for fluorescence
• storage temp.: −20°C
• SMILES string: [O-]Cl(=O)(=O)=O.CN(C)c1ccc2C=C3C=C\C(C=C3N(CCCC(=O)ON4C(=O)CCC4=O)c2c1)=[N+](\C)C; [O-]Cl(=O)(=O)=O.CN(C)c1ccc2C=C3C=C\C(C=C3N(CCCC(=O)ON4C(=O)CCC4=O)c2c1)=[N+](\C)C
• InChI: 1S/C25H29N4O4.ClHO4/c1-26(2)19-9-7-17-14-18-8-10-20(27(3)4)16-22(18)28(21(17)15-19)13-5-6-25(32)33-29-23(30)11-12-24(29)31;2-1(3,4)5/h7-10,14-16H,5-6,11-13H2,1-4H3;(H,2,3,4,5)/q+1;/p-1
• InChI key: QYNYOHCMTHAQIE-UHFFFAOYSA-M

Description

Application

Atto 495 is a new label for proteins and oligo-nucleotides with high molecular absorption (80.000) and quantum yield (0.45) as well as sufficient stoke′s shift (excitation maximum 495 nm, emission maximum 527 nm). Atto 495 is characterized by a high photostability and thermostability. It is slightly hydrophilic, and solubility in polar solvents as DMF or DMSO is high.

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Other Notes

Incorporation in a zeolite photonic nanodevice

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves