19924

Pyroglutamate Aminopeptidase from Pyrococcus furiosus, recombinant from E. coli

7-13 mU (per vial)

• Synonym(s): Pyrase, Pyroglutamyl-Peptidase I, Pyrrolidone Carboxyl Peptidase
• CAS Number: 9075-21-2
• EC Number: 3.4.19.3 (BRENDA, IUBMB)
• EC Number: 232-981-8
• MDL number: MFCD00132139
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• recombinant: expressed in E. coli
• Quality Level: 100
• form: lyophilized
• specific activity: 7-13 mU (per vial)
• mol wt: M_r ~28000
• storage temp.: −20°C

Description

Packaging

package of 0.01 Unit

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol Pyroglutamate-p-nitroanilide per minute at pH 7.0 and 37 °C

Analysis Note

enzyme activity: the optimum temperature is 95-100 °C (the enzyme is stable up to 75 °C), the optimum pH is 6-9 (stable from pH 6-9). Inhibitors: PCMB, Hg⁺.

Other Notes

An enzyme that removes pyroglutamic acid (pGlu) from pGlu-peptide and proteins; employed in Edman degradation.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H315,H319,H334,H335
• Precautionary Statements: P261 - P305 + P351 + P338 - P342 + P311
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable