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Quality Level
fluorescence
λex 503 nm; λem 610 nm±20 nm in 0.1 M bicarbonate buffer pH 8.3
storage temp.
−20°C
Related Categories
General description
Chromeo P503 labels proteins and peptides by exhibiting a color change from blue to red upon binding to primary amines. Chromeo P503 displays a weak fluorescence with a quantum yield <1% in solution. After conjugation to a primary amine group, the label undergoes a shortwave spectral shift of >100 nm and the quantum yield rises to 50%. This property allows a distinct detection of primary amines, proteins, and other biomolecules.
Application
Chromeo P503 is used as a fluorogenic reagent to label primary amine groups within molecules such as proteins. Chromeo P503 labeled proteins may be studied in a variety of gel electrophoresis and chromatographic applications.
Caution
To ensure stability, the lyophilized dye should be stored at 4°C in the dark. This product is guaranteed for 6 months from the date of arrival.
Legal Information
Chromeo is a trademark of Active Motif Chromeon GmbH
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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