Skip to Content
Merck
All Photos(1)

Key Documents

37294

Sigma-Aldrich

Dihydrofolate Reductase from bovine liver

~8 U/mL, ammonium sulfate suspension, off-white

Synonym(s):

Tetrahydrofolate Dehydrogenase

Sign Into View Organizational & Contract Pricing


About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bovine liver

Quality Level

form

ammonium sulfate suspension

specific activity

≥6 U/mg protein

concentration

~8 U/mL

color

off-white

density

1.2 g/mL at 20 °C

storage temp.

2-8°C

Application

Dihydrofolate reductase (DHFR) is involved in the synthesis of purines, thymidine and glycine in folate metabolism. DHFR, from bovine liver, is used for methotrexate analysis . DHFR is useful to study various cancers such as sarcomas, leukemias and head and neck cancers .

Biochem/physiol Actions

Dihydrofolate reductase (DHFR) reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as an electron donor. The binding of antitumor agents, such as methotrexate, to DHFR prevents the formation of reduced folates, which are essential for DNA synthesis .

Unit Definition

1 U corresponds to the amount of enzyme which converts 1 μmol 7,8-dihydrofolate and NADPH to 5,6,7,8-tetrahydrofolate and NADP per minute at pH 6.5 and 25 °C

Physical form

Suspension in 3.6 M ammonium sulfate solution, pH 7.0

Other Notes

In folate metabolism for the synthesis of purines, thymidine and glycine
Sales restrictions may apply

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service