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Sigma-Aldrich

Atto 647N DOPE

suitable for fluorescence

Synonym(s):

1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine labeled with Atto 647N

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About This Item

UNSPSC Code:
12352116
NACRES:
NA.32
Pricing and availability is not currently available.
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Assay

≥80.0% (HPCE)
≥90.0% (HPLC)

Quality Level

100

manufacturer/tradename

ATTO-TEC GmbH

λ

in chloroform/methanol

UV absorption

λ: 643.0-649.0 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

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Tantalum foil, 50x50mm, thickness 2.0mm, annealed, 99.9%

GF38824476

Tantalum

Tantalum foil, 50x50mm, thickness 1.0mm, annealed, 99.9%

GF10151873

Tantalum

assay

≥99.9%

assay

99.9%

assay

99.9%

assay

≥99.9%

manufacturer/tradename

Goodfellow 883-387-30

manufacturer/tradename

Goodfellow 500-365-10

manufacturer/tradename

Goodfellow 388-244-76

manufacturer/tradename

Goodfellow 101-518-73

form

foil

form

foil

form

foil

form

foil

mp

2996 °C (lit.)

mp

2996 °C (lit.)

mp

2996 °C (lit.)

mp

2996 °C (lit.)

density

16.69 g/cm3 (lit.)

density

16.69 g/cm3 (lit.)

density

16.69 g/cm3 (lit.)

density

16.69 g/cm3 (lit.)

bp

5425 °C (lit.)

bp

5425 °C (lit.)

bp

5425 °C (lit.)

bp

5425 °C (lit.)

General description

Atto 647N belongs to a new generation of fluorescent labels for the red spectral region. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, excellent fluorescence quantum yield, high photostability, excellent ozone resistance, good solubility, and very little triplet formation. Atto 647N is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1.
In common with most Atto-labels, absorption and fluorescence are independent of pH in the range of 2 to 11, used in typical applications. As supplied Atto 647N consists of a mixture of two isomers with practically identical absorption and fluorescence properties.

Atto-Dye Labeled Phospholipids

Sigma-Aldrich offers a variety of glycero-phospholipids carrying one or two fatty acid groups (lipophilic groups) and a phosphate ester residue (hydrophilic group). They are labeled at the hydrophilic head group. After incorporation of the phospholipid into a membrane the fluorophore is located at the water/lipid interface of the membrane. We currently provide 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), palmitoyl-sn-glycero-phosphoethanolamine (PPE), and 1,2-dimyristoyl-sn-glycero-3-phospho-ethanolamine (DMPE) labeled with Atto-dyes.

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Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK5529
BCCJ9342
BCCJ6664
BCCJ0120
BCCH8459

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Hitomi Komura et al.
iScience, 13, 452-477 (2019-03-05)
We identified ∼30-mer amyloid-β protein (Aβ) assemblies, termed amylospheroids, from brains of patients with Alzheimer disease (AD) as toxic entities responsible for neurodegeneration and showed that Na+,K+-ATPase α3 (NAKα3) is the sole target of amylospheroid-mediated neurodegeneration. However, it remains unclear

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