A1765

S-Acetyl-coenzyme A synthetase from baker′s yeast (S. cerevisiae)

lyophilized powder, ≥3 units/mg protein

• Synonym(s): Acetate CoA ligase (AMP forming), Acetate thiokinase
• CAS Number: 9012-31-1
• EC Number: 6.2.1.1 (BRENDA, IUBMB)
• EC Number: 232-729-7
• MDL number: MFCD00081267
• UNSPSC Code: 12352204
• NACRES: NA.26

Properties

• form: lyophilized powder
• Quality Level: 200
• specific activity: ≥3 units/mg protein
• composition: Protein, 10-30% biuret
• storage temp.: −20°C

Description

Application

S-Acetyl-coenzyme A synthetase from baker′s yeast (S. cerevisiae) has been used in the synthesis of adenosine 5′-tetraphosphate and adenosine 5′-pentaphosphate.

S-Acetyl-coenzyme A synthetase may be used to study various metabolic pathways, such as glycolysis, gluconeogenesis, pyruvate metabolism and CO₂ fixation. It may also be used in gene expression studies.

Biochem/physiol Actions

Acetyl-coenzyme A synthetase catalyzes the production of acetyl-CoA. It is involved in histone acetylation in the nucleus. It may be involved in the growth of nonfermentable carbon sources such as glycerol. Acetyl-coenzyme A synthetase is induced by acetate, acetaldehyde and ethanol .

Packaging

Package size based on protein content.

Unit Definition

One unit will form 1.0 μmole of S-acetyl coenzyme A from acetate, ATP, and coenzyme A per min at pH 7.5 at 37 °C.

Physical form

Lyophilized powder containing stabilizers as potassium phosphate, sucrose, and reduced glutathione

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves