B2787
Biotinylated Molecular Weight Marker
mol wt 6,500-180,000 Da
Synonym(s):
protein markers, protein molecular weight markers, protein standards
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About This Item
UNSPSC Code:
12352200
eCl@ss:
34058011
NACRES:
NA.25
Recommended Products
form
lyophilized powder
Quality Level
usage
1 vial sufficient for 200 applications (on 10 cm × 10 cm mini gels)
mol wt
6,500-180,000 Da
packaging
vial of 0.1 mg protein (also contains 0.1 mg NaCl and 0.1 mg sucrose)
storage temp.
−20°C
Related Categories
Application
For SDS-PAGE and protein transfer, the Biotinylated Molecular Weight Standard Mixture contains proteins, which have been biotin conjugated for use as SDS-PAGE standards for the estimation of the molecular weights of blotted proteins. Visualization is accomplished using streptavidin-peroxidase (Cat. No. S2438) and a color development reagent such as 4-Chloro-1-naphthol (Cat. No. C8890), eliminating the need to cut out separate lanes for general protein stains and can be performed simultaneously with immunostaining procedures. These standards may be used with either the Laemmli or the Weber and Osborn SDS-PAGE procedures.
Components
This product contains:
- α2-macroglobulin (equine serum)
- β-galactosidase (E. coli)
- phosphorylase b (rabbit muscle)
- catalase (bovine liver)
- alcohol dehydrogenase (yeast)
- carbonic anhydrase (bovine erythrocyte)
- trypsin inhibitor (soy bean)
- lysozyme (chicken egg)
- aprotinin (bovine lung)
Other Notes
Contains nine biotinylated proteins (approx 0.1 mg total).
Reconstitution
Each vial is reconstituted with 1 mL of 1x sample buffer (Cat. No. S3401) and incubated for 2-3 minutes at 100 °C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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