B3670

Betacellulin human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): BTC
• CAS Number: 163150-12-7
• MDL number: MFCD00676521
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• Product Name: Betacellulin human, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)
• biological source: human
• Quality Level: 100
• recombinant: expressed in E. coli
• Assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.1-0.6 ng/mL ED₅₀
• mol wt: protein 9.5 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P35070
• storage temp.: −20°C
• Gene Information: human ... BTC(685)

Description

Application

This product has been used to study differentiation of pancreatic acinar cell line (AR42J) into insulin-secreting cells and has been linked to a role in the growth and differentiation of islet cells in human pancreas.

Biochem/physiol Actions

Betacellulin, and the other members of the EGF family of growth factors, binds with high affinity to both EGFR and to erbB-4. Overexpression of Betacellulin with erbB-3 and erbB-4 has been linked to human endometrial adenocarcinoma.

Caution

This product should be stored at -20°C. After preparation, the product can be stored at 2-8°C for one month, or frozen in aliquots at -20°C or -70°C for more extended storage.

Preparation Note

This product was lyophilized from a 0.2 μm filtered solution of PBS containing 5 mg of bovine serum albumin. It should be reconstituted by adding sterile PBS containing 0.1% HAS or BSA to a concentration greater than 1 μg/mL.

Analysis Note

The biological activity of this product is measured by an ability to stimulate H-thmidine incorporation in EGF-responsive mouse BALB/c 3T3 fibroblasts. The EC₅₀ is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves