Skip to Content
Merck

Key Documents

View All Documentation

Skip To

C0888

Sigma-Aldrich

Clostripain from Clostridium histolyticum

≥20 units/mg solid

Synonym(s):

Clostridiopeptidase B, Proteinase from Clostridium histolyticum

Sign Into View Organizational & Contract Pricing

About This Item

CAS Number:
9028-00-6
EC Number:
3.4.22.8 (BRENDA, IUBMB)
EC Number:
232-822-2
MDL number:
MFCD00130814
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

form

lyophilized powder

Quality Level

100

specific activity

≥20 units/mg solid

mol wt

15.4 kDa
 41.7 kDa

impurities

salt, essentially free

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
639086629412540
1,2,3-Trimethylbenzene analytical standard

45935

1,2,3-Trimethylbenzene

Mesitylene analytical standard

63908

Mesitylene

Methylcyclohexane analytical standard

66294

Methylcyclohexane

Benzene analytical standard

12540

Benzene

application(s)

environmental

application(s)

environmental

application(s)

environmental

application(s)

environmental

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

format

neat

format

neat

format

neat

format

neat

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

grade

analytical standard

grade

analytical standard

grade

analytical standard

grade

analytical standard

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

Application

Clostripain from Clostridium histolyticum has been used as a proteolytic enzyme in perfusate to detect its effect on tube hematocrit.[1] It has also been used in limited proteolysis of DNA polymerase (gp43) of phage T4 (RB69 gp43).[2]

Biochem/physiol Actions

Clostripain from Clostridium histolyticum is composed of two polypeptide chains, with molecular masses of 41.7 kDa and 15.4 kDa. Clostripain has a highly restricted substrate specificity for Arg-Xaa peptide bonds. Therefore, clostripain has been explored as a potential enzyme for protein sequencing purposes. It has also been studied as a catalyst for condensation of pharmaceutically important peptides containing Arg-Pro bonds.[3]

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Quality

Purified, essentially salt-free

Unit Definition

One unit will hydrolyze 1.0 μmole of BAEE per min at pH 7.6 at 25 °C in the presence of 2.5 mM DTT.

Analysis Note

The enzyme must be activated for 2-3 hours before use by dissolving in 2.5 mM dithiothreitol containing 1.0 mM calcium acetate.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H334,H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    BCCK2455
    BCCJ2943
    BCCH9520
    BCCH4761
    BCCG5867

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Search for a COA

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Z Korsak et al.
    International journal of occupational medicine and environmental health, 9(4), 341-349 (1996-01-01)
    Neurotoxic effects of trimethylbenzene isomers (pseudocumene, mesitylene and hemimellitene) in male rats were investigated in conditions of acute and subchronic inhalation exposure. Rotarod performance and pain sensitivity behaviour were tested in rats exposed to trimethylbenzenes at concentrations of 250-2,000 ppm
    Z Korsak et al.
    International journal of occupational medicine and environmental health, 10(3), 303-311 (1997-01-01)
    Sensory respiratory irritation effects of trimethylbenzene isomers (TMBs) (hemimellitene, mesitylene and pseudocumene) in male Balb/C mice were investigated in conditions of acute exposure and in male Wistar rats in conditions of repeated 90-day inhalation exposure to pseudocumene. The pseudocumene, mesitylene
    Z Korsak et al.
    International journal of occupational medicine and environmental health, 13(3), 223-232 (2000-12-08)
    Toxic effects of exposure to 1,2,3-trimethylbenzene (hemimellitene) in the condition of subchronic inhalation experiment were examined. Rats were exposed to vapours of hemimellitene at concentrations of 123 mg/m3, 492 mg/m3 and 1230 mg/m3, 6 h/day, 5 days/week for 3 months.
    Y Tsujimoto et al.
    Chemosphere, 39(5), 725-730 (1999-08-17)
    The structure was investigated of the mercapturic acid excreted in urine of rats after the i.p. administration of 1,2,3-trimethylbenzene. Of the two regioisomeric mercapturic acids, i.e. N-acetyl-S-(2,3-dimethylbenzyl)-L-cysteine and N-acetyl-S-(2,6-dimethyl-benzyl)-L-cysteine, only the former was isolated by preparative HPLC and identified, by
    Application of gas chromatography in conjunction with enrichment of samples on active carbon to determine low concentrations of organic compounds in air. Part I. Aromatic hydrocarbons.
    H Swedrowska et al.
    Bulletin of the Institute of Maritime and Tropical Medicine in Gdynia, 37(1-2), 103-112 (1986-01-01)
    View All Related Papers

    Protocols

    GC Analysis of Hydrocarbons in Gasoline on Petrocol® DH

    Protocol for GC Analysis of Hydrocarbons in Gasoline on Petrocol® DH

    GC Analysis of Hydrocarbons in Gasoline on Equity®-1 (15 m x 0.10 mm I.D., 0.10 μm), Fast GC Analysis

    -Xylene; Nonane; Propylbenzene; Mesitylene; 1,2,4-Trimethylbenzene; 1,2,3-Trimethylbenzene; 1,3-Diethylbenzene; 1,4-Dimethyl-2-ethylbenzene; 1,2-Dimethyl-4-ethylbenzene; Durene; 1,2,3,5-Tetramethylbenzene; 1,2,3,5-Tetramethylbenzene; 2-Methylnaphthalene (β)

    GC Analysis of Hydrocarbons in Gasoline on Petrocol® DH, Isothermal

    GC Analysis of Hydrocarbons in Gasoline on Petrocol® DH, Isothermal

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service