C4483

CD152/Fc Chimera, Cytolytic from mouse

recombinant, expressed in NS.1 cells, buffered aqueous solution

• Synonym(s): CTLA4/Fc Chimera, cytolytic
• MDL number: MFCD01092888
• UNSPSC Code: 51111800
• NACRES: NA.77

Properties

• biological source: mouse
• Quality Level: 200
• recombinant: expressed in NS.1 cells
• Assay: ≥99% (SDS-PAGE)
• form: buffered aqueous solution
• mol wt: 97 kDa
• packaging: pkg of 1 mg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: ≤1 EU/mg protein Endotoxin level (LAL test)
• UniProt accession no.: P09793
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... Cd152(12477), Ctla4(12477)

Description

General description

The complement (C1q) and FcγR I binding sites of the Fcγ2a fragment allow the Fc portion of the fusion protein to effectively facilitate direct antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).

Biochem/physiol Actions

CD152 (CTLA4), a cell surface glycoprotein expressed at low levels on activated T cells, is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2). A related cell surface glycoprotein, CD28, binds to CD80 and CD86 with lower affinity. The soluble CD152/Fc chimeric fusion protein blocks the B7/CD28 signaling pathway by binding to CD80 and CD86.

Other Notes

The extracellular domain (160 amino acids) of mouse CD152 is fused to mouse IgG2a Fc domain.

Physical form

Solution, 0.2 μm filtered, in phosphate buffered saline, pH 7.4, with no preservative added.

Preparation Note

Purified from serum-free tissue culture supernatant

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves