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C7240

Sigma-Aldrich

Anti-phospho-c-Met (pTyr1230/pTyr1234/pTyr1235) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.44

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

rat, human, mouse

technique(s)

western blot: 1:1000 using using 293T kidney cells transiently transfected with human c-Met and stimulated with HGF

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pTyr1230/pTyr1234/pTyr1235)

Gene Information

human ... MET(4233)
mouse ... Met(17295)
rat ... Met(24553)

General description

MET, a gene for receptor tyrosine kinase, was first isolated in human osteosarcoma-derived cell line. Hepatocyte growth factor (HGF) has been identified as the natural ligand for the c-Met receptor protein. HGF-induced c-Met activation is tightly regulated in the cells. Activation of the c-Met receptor results in binding and/or phosphorylation of many intracellular signaling proteins including multiple adaptor proteins (e.g., Grb2, Shc, Cbl, Crk, cortactin, paxillin, and GAB1) and a variety of other signal transducers (e.g., PI 3-kinase, FAK, Src, ERK1, JNK1, PLCa, and STAT3). HGF/Met signalling is actively responsible stimulating tumor angiogenesis, tumor growth, hypoxia and metastasis. Met overexpression through persistent ligand stimulation has been reported in many epithelial and mesenchymal cancers. Overexpression of Met enables metastasis in lung cancers, mutations in MET gene sequence have been reported in papillary renal cell carcinoma, lung cancers, ovarian, prostate, cervical, gastric and head and neck cancers. Anti-phospho-c-Met recognises human and mouse c-Met phosphorylated on Tyr1230, Tyr1234 and Try1235. Cross reaction with rat is expected due to 100% homology.

Immunogen

synthetic phosphopeptide derived from the region of c-Met that contains tyrosines 1230, 1234 and 1235.

Application

Detection of phospho-c-Met by immunoblotting may be performed by using a working concentration of 0.11.0 μg/mL in 293T kidney cells, SP-1 mouse myeloma cells stimulated with HGF and A431 cells stimulated with EGF.

Physical form

Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, with 50% glycerol, 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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