C8218
Monoclonal Anti-Cdk4 antibody produced in mouse
clone DCS-31, ascites fluid
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About This Item
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
DCS-31, monoclonal
mol wt
antigen 33 kDa
contains
15 mM sodium azide
species reactivity
rat, mouse, human
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:1,000 using a cultured human tumor cell line extract
General description
Cdk4 exists, in part, as a multi-protein complex with a D-type cyclin, proliferating cell nuclear antigen (PCNA) and a protein inhibitor, p21Cip1. Cdk4 associates separately with p16, particularly in cells lacking a functional retinoblastoma protein.
Monoclonal Anti-Cdk4 (mouse IgG2a isotype) is derived from the DCS-31 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Cyclin-dependent kinase 4 is localized in human chromosome 12q14.1.
Specificity
The antibody reacts specifically with Cdk4 and does not recognize other Cdk types.
Immunogen
recombinant human Cdk4 protein
Application
Monoclonal Anti-Cdk4 antibody was used in:
- immunoprecipitation of human melanoma cell line.
- immunofluorescence of fibrosarcoma cells.
- western blot analysis
- immunofluorescence
- immunohistochemistry
Biochem/physiol Actions
In association with cyclins, cyclin-dependent kinases (CDKs) forms active kinase complexes which is responsible for regulating cell cycle progression in eukaryotic cells. Within the complexes, the CDKs serves a catalytic protein kinase activity. This catalytic activity is regulated by two mechanisms: protein phosphorylation and association of regulatory subunits.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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