Sf 1Ep

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 90092702, rabbit skin, Epithelial

• UNSPSC Code: 41106514

Properties

• biological source: rabbit skin
• packaging: tube of 5 μg 90092702-DNA-5UG; pkg of vial of cells 90092702-1VL
• growth mode: Adherent
• karyotype: 2n = 42
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Rabbit epidermis, cottontail

Cell Line Description

From minced ear epidermis of adult male cottontail rabbit with papillomatosis. Cells can be cultured continuously at pH 7.4 and 37°C but may also be adequately maintained at 25°C. Developed for studies of epidermis specific virus replication. Also know as Sf1Ep (NBL-11).

Application

Virus replication studies (may be useful for viruses producing lesions in skin)

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Finite lifespan. Maximum of 80 passages expected. Cells become spindle shaped when confluent.

Other Notes

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