D0690

DNA Gyrase from Escherichia coli

aqueous glycerol solution

• EC Number: 5.99.1.3 (BRENDA, IUBMB)
• MDL number: MFCD00211877
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: Escherichia coli
• Quality Level: 200
• form: aqueous glycerol solution
• mol wt: ~374 kDa
• concentration: ≥2 unit/μL
• technique(s): cell based assay: suitable
• UniProt accession no.: P0AES4; P0AES6
• application(s): cell analysis
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: Escherichia coli K12 ... gyrA(946614), gyrB(948211)

Description

Application

DNA gyrase from Escherichia coli has been used in a study to investigate a comparative proteomic approach to better define Deinococcus nucleoid specificities. DNA gyrase from Escherichia coli has also been used in a study to investigate the role of the DnaK-ClpB bichaperone system in DNA gyrase reactivation.

Biochem/physiol Actions

Can be used to supercoil plasmids.

DNA gyrase is supplied as an A₂B₂ holoenzyme. The molecular mass of subunit A is 97 kDa and that of subunit B is 90 kDa. It catalyzes the ATP-dependent introduction of negative supercoils into relaxed DNA. DNA gyrase has been successfully converted into a type II topoisomerase by mutagenesis experiments.

Unit Definition

One unit of gyrase activity will supercoil 0.5 micrograms of pBR-322 DNA in 30 minutes at 37 °C.

Other Notes

Solution in 50% Glycerol containing Tris buffer, DTT and EDTA.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable