D0690
DNA Gyrase from Escherichia coli
aqueous glycerol solution
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Recommended Products
biological source
Escherichia coli
Quality Level
form
aqueous glycerol solution
mol wt
~374 kDa
concentration
≥2 unit/μL
technique(s)
cell based assay: suitable
application(s)
cell analysis
shipped in
dry ice
storage temp.
−70°C
Gene Information
Escherichia coli K12 ... gyrA(946614), gyrB(948211)
Application
DNA gyrase from Escherichia coli has been used in a study to investigate a comparative proteomic approach to better define Deinococcus nucleoid specificities. DNA gyrase from Escherichia coli has also been used in a study to investigate the role of the DnaK-ClpB bichaperone system in DNA gyrase reactivation.
Biochem/physiol Actions
Can be used to supercoil plasmids.
DNA gyrase is supplied as an A2B2 holoenzyme. The molecular mass of subunit A is 97 kDa and that of subunit B is 90 kDa. It catalyzes the ATP-dependent introduction of negative supercoils into relaxed DNA. DNA gyrase has been successfully converted into a type II topoisomerase by mutagenesis experiments.
Unit Definition
One unit of gyrase activity will supercoil 0.5 micrograms of pBR-322 DNA in 30 minutes at 37 °C.
Other Notes
Solution in 50% Glycerol containing Tris buffer, DTT and EDTA.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service