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D4168

Sigma-Aldrich

SIGMAFAST™ 3,3′-Diaminobenzidine tablets

SIGMAFAST™, tablet

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About This Item

CAS Number:
91-95-2
MDL number:
MFCD00007725
UNSPSC Code:
12352204
PubChem Substance ID:
24893827
NACRES:
NA.83

Product Name

SIGMAFAST™ 3,3′-Diaminobenzidine tablets, tablet, To prepare 1 mL

Quality Level

200

form

tablet

solubility

deionized water: 1 mL, clear, pink

storage temp.

−20°C

SMILES string

Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2

InChI key

HSTOKWSFWGCZMH-UHFFFAOYSA-N

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Application

Developed for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST™ DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packets

Reconstitution

Each tablet set dissolved in 1 mL deionized water yields a ready-to-use buffered solution containing DAB and urea hydrogen peroxide.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Classifications

Carc. 1B - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Ox. Liq. 3 - Skin Irrit. 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

5.1B - Oxidizing hazardous materials

Flash Point(F)

53.6 °F - closed cup

Flash Point(C)

12 °C - closed cup

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Certificates of Analysis (COA)

Lot/Batch Number
SLBV8376
SLBP9645V
SLBM4338V
SLBG6151V
SLBG6151

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Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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