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F4051

Sigma-Aldrich

Proteasome Fraction II from rabbit

5 mg/mL, Reticulocyte cell extract, solution

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About This Item

MDL number:
MFCD03458197
UNSPSC Code:
12352200
NACRES:
NA.77

Quality Level

200

form

solution

concentration

5 mg/mL

storage temp.

−70°C

General description

Manufactured for Sigma by Boston Biochem., Inc.

Application

Proteasome Fraction II is ideal for demonstrating ubiquitin- and ATP-dependent degradation/conjugation of radiolabeled or immunodetectable substrates. Isopeptidase inhibitor and a proteasome inhibitor are highly recommended for the accumulation of ubiquitin protein conjugates. Typical assay concentration for FII is 0.5 - 4 mg/ml.

Biochem/physiol Actions

Essentially ubiquitin and ATP free. Contains E1, most E2s, UCHs, and the proteasome 20S and 26S isoforms.
Protein fraction of a cell extract that binds to anion-exchange resin.

Physical form

Solution in 25 mM HEPES, pH 7.6.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLBD8071
SLBD0228
SLBC3951
124K2710
014K2719

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