G1512

β-Glucuronidase from Helix pomatia

Type H-5, lyophilized powder, ≥400,000 units/g solid

• Synonym(s): β-D-Glucuronide glucuronosohydrolase
• CAS Number: 9001-45-0
• EC Number: 3.2.1.31 (BRENDA, IUBMB)
• EC Number: 232-606-8
• MDL number: MFCD00130629
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• type: Type H-5
• Quality Level: 200
• form: lyophilized powder
• specific activity: ≥400,000 units/g solid
• secondary activity: ≤40,000 units/g solid sulfatase
• storage temp.: −20°C

Description

General description

β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Application

β-Glucuronidase from Helix pomatia has been used:

• in the enzymatic treatment of urine samples for liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) analysis
• in the quercetin extraction procedure from pig or rat tissues for high performance liquid chromatography (HPLC) analysis
• in the enzymatic deconjugation step in urine and plasma sample preparation for gas chromatography-mass spectrometry analysis (GC-MS)

β-glucuronidase was used to hydrolyze conjugated glucuronide and sulfate metabolites.

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Biochem/physiol Actions

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Quality

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Linkage

A further purification of G0876 by gel permeation chromatography.

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H317,H334
• Precautionary Statements: P261 - P272 - P280 - P284 - P302 + P352 - P304 + P340 + P312
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves