G6751

α-Glycerophosphate Dehydrogenase from rabbit muscle

Type I, ammonium sulfate suspension, 100-300 units/mg protein

• Synonym(s): sn-Glycerol-3-phosphate Dehydrogenase from rabbit muscle, sn-Glycerol-3-phosphate:NAD⁺ 2-oxidoreductase
• CAS Number: 9075-65-4
• EC Number: 1.1.1.8 (BRENDA, IUBMB)
• EC Number: 232-982-3
• MDL number: MFCD00130487
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: rabbit muscle
• Quality Level: 200
• type: Type I
• form: ammonium sulfate suspension
• specific activity: 100-300 units/mg protein
• storage condition: (Tightly closed)
• technique(s): activity assay: suitable
• color: white
• foreign activity: Lactic dehydrogenase, pyruvate kinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%; Triosephosphate isomerase ≤0.02%
• storage temp.: 2-8°C

Description

General description

α-Glycerophosphate dehydrogenase is a membrane protein and a flavin-linked primary dehydrogenase.

Research area: Cell Signaling

Application

α-Glycerophosphate Dehydrogenase from rabbit muscle has been used:

• in the reaction mixture to measure the glycerol kinase activity
• to demonstrate compartmentalized enzymatic reactions where NADH is involved
• in the reaction mixture assay for L-fuculose-1-phosphate aldolase

Biochem/physiol Actions

α-Glycerophosphate dehydrogenase catalyzes the oxidation of L-α-glycerophosphate (Glp) to form dihydroxyacetone phosphate (DHAP) and hydrogen peroxide (H2O2). This enzyme is associated with respiratory electron transport chain, glycolysis, and phospholipid metabolism.

Unit Definition

One unit will convert 1.0 μmole of dihydroxyacetone phosphate to α-glycerophosphate per min at pH 7.4 at 25 °C.

Physical form

Crystalline suspension in 3.2 M (NH₄)₂SO₄ and 0.1 g/L EDTA solution, pH 6.0

Preparation Note

Prepared by modification of the method of Fondy.

Analysis Note

Protein determined by biuret.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable