H6916

Macrophage Colony-Stimulating Factor human

≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

• Synonym(s): MCSF
• MDL number: MFCD00166591
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Product Name: Macrophage Colony-Stimulating Factor human, M-CSF, recombinant, expressed in HEK 293 cells, suitable for cell culture
• biological source: human
• Quality Level: 100
• recombinant: expressed in HEK 293 cells
• Assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• potency: ≤4 ng/mL EC₅₀
• quality: endotoxin tested
• mol wt: dimer 35-40 kDa (glycosylated)
• packaging: pkg of 10 μg
• manufacturer/tradename: HumanZyme
• technique(s): cell culture | mammalian: suitable
• UniProt accession no.: P09603
• storage temp.: −20°C
• Gene Information: human ... CSF1(1435)

Description

General description

M-CSF is encoded by the gene mapped to human chromosome 1p13.3. It is a glycosylated polypeptide, which exists as a homodimer. M-CSF is produced by macrophage, fibroblasts, lymphocyte and endothelial cells.

Biochem/physiol Actions

Macrophage CSF (M-CSF) as a growth factor, stimulates the proliferation and differentiation of monocyte/macrophage-lineage cells. It also boosts antibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages. In addition, human M-CSF inhibits bone resorption by osteoclasts. It might also play a crucial role in atherosclerosis. Overexpression of the gene has been observed in Paget′s disease of bone (PDB).

Physical form

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells (mouse myeloid Leukemia indicator cell line).

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable