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Key Documents

H8283

Sigma-Aldrich

Hemocyanin from Megathura crenulata (keyhole limpet)

PBS solution

Synonym(s):

KLH, Keyhole limpet hemocyanin

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.25

biological source

Megathura crenulata

Quality Level

form

PBS solution

concentration

3.0-7.0 mg/mL protein (A280)

copper content

0.10-0.30% (based on protein)

technique(s)

ELISA: suitable

storage temp.

2-8°C

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General description

Gastropod hemocyanins are massive glycoproteins (4 to 8 MDa) designed by an intricate arrangement of 10 subunits that are self-assembled into hollow cylinders 35 nm in diameter.
Hemocyanin is a high molecular weight copper containing glycoprotein. It reversibly binds oxygen and forms the extra-cellular respiratory protein of mollusks. Keyhole limpet hemocyanin (KLH) is filtered from the hemolymph of Megathura crenulata, also called the giant keyhole limpet. It is native to the southern California coast and Mexico.

Application

Keyhole limpet hemocyanin (KLH) is a strong and well known immune-stimulant in both experimental animals and humans. It has been used as a hapten carrier protein to enhance antigen-specific T cell priming and is known to stimulate a CD4+ T-cell response.
Hemocyanin was used to study if antipeptide antibodies specifically recognize the l1 protein of the human papilloma virus of type 31. It was also used to study the acid-induced unfolding of didecameric keyhole limpet hemocyanin.

Physical form

Solution in PBS, pH 7.2, containing 1mM CaCl2 and 0.5 mM MgCl2 with 15 mM sodium azide as preservative

Preparation Note

Chromatographically purified.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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