H8891
Heparinase III from Flavobacterium heparinum
Lyophilized powder stabilized with approx. 25% (w/w) bovine serum albumin, ≥30 units/mg protein (enzyme + BSA)
Synonym(s):
Heparin Lyase III, Heparitinase I, Heparitinase from Flavobacterium heparinum
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
biological source
bacterial (Flavobacterium heparinum)
Quality Level
conjugate
conjugate (Glucosaminoglycan)
form
lyophilized powder
specific activity
≥30 units/mg protein (enzyme + BSA)
mol wt
70.8 kDa
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
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Application
Heparinase III from Flavobacterium heparinum has been used to cleave specific GAGs. It has been used to study its effect on the electrophoretic mobility of the protein reacting with the anti-aFGF (acidic fibroblast growth factor) antibody.
Biochem/physiol Actions
Heparin-degrading lyase that recognizes heparin sulfate proteoglycan as its primary substrate.
Heparinase III prevents the formation of new blood vessels in vivo. It specifically degrades heparin sulphate at the HNY-I and HNY,6X-G2 links. Unlike other heparinise, heparinase III does not contain the amino acid cysteine. It contains 13 histidine residues, which is responsible for its catalytic action. This enzyme is not active towards heparin or low molecular weight heparins.
Unit Definition
One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units. Package sizes are sold in Sigma units.
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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