H9034

Hepatitis C virus core antigen

~1 mg/mL, ≥95% (SDS-PAGE), recombinant, expressed in E. coli

• MDL number: MFCD02264926
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• Assay: ≥95% (SDS-PAGE)
• mol wt: 22 kDa (plus 114 kDa galactosidse)
• concentration: ~1 mg/mL
• UniProt accession no.: G9G988; L7S4V3
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: hepatitis C virus ... HCVgp1(951475) ; human ... HBVgp4(944568)

Description

General description

Hepatitis C virus core antigen (HCV-cAg) is a well-preserved antigenic protein of the internal capsid.[1]

Hepatitis C virus glycoprotein 1 (HCVgp1), also referred to as polyprotein, is crucial for the induction of double-membrane vesicles, sites of HCV RNA amplification. The amino terminal of polyprotein contains the structural proteins core, envelope protein 1 (E1) and E2.[2] The proteolytic cleavage of polyprotein results in the formation of 10 mature viral proteins which are needed for viral replication and assembly.[3]

amino acids 2-192 of HCV polyprotein

Specificity

Reacts strongly with human HCV positive serum.

Application

Hepatitis core antigen (HCA) has been used as an analyte in enzyme-linked immunosorbent assay (ELISA) to study the mechanism of bovine serum albumin (BSA) blocking in common ELISA protocols.[4]

Biochem/physiol Actions

Hepatitis C virus core antigen (HCV-cAg) may be a less expensive way to diagnose active hepatitis C virus (HCV) infection in the hemodialysis (HD) population.[5]

Positive control for HCV antibodies.

Other Notes

HCV core antigen [2-192]-Galactosidase-tagged at the N-terminus.

Physical form

Solution in 8 M Urea, 20 mM Tris-HCl, pH 8.0, 10 mM 2-mercaptoethanol.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable