I0406

IL-6 rat

>97% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture

• Synonym(s): Interleukin-6 from rat
• MDL number: MFCD01323739
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Product Name: Interleukin-6 from rat, IL-6, recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture
• biological source: rat
• Quality Level: 200
• recombinant: expressed in E. coli
• Assay: >97% (SDS-PAGE)
• form: buffered aqueous solution
• potency: 0.01-0.06 ng/mL ED₅₀
• quality: endotoxin tested
• mol wt: predicted mol wt ~22 kDa
• packaging: pkg of 10 and 50 μg
• technique(s): cell culture | mammalian: suitable
• impurities: <0.01EU/mg (LAL test)
• UniProt accession no.: P20607.1
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: rat ... Il6(24498)

Description

Biochem/physiol Actions

Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-β-2 (IFN-β₂), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.

Physical form

Supplied as a 0.2 μm filtered solution in 50 mM sodium acetate, pH 4, with BSA as a carrier protein.

Preparation Note

Recombinant, rat Interleukin-6 is produced from a DNA sequence encoding the mature rat IL-6 protein. The methionyl form of mature rat IL-6, a 188 amino acid protein, has a predicted molecular mass of approx. 22 kDa. Rat and mouse IL-6 share 93% amino acid sequence identity.

Analysis Note

The biological activity is measured in a cell proliferation assay using IL-6 dependent mouse plasmacytoma cell line, T1165.85.2.1.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable