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I0406

Sigma-Aldrich

IL-6 rat

>97% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture

Synonym(s):

Interleukin-6 from rat

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About This Item

MDL number:
UNSPSC Code:
12352202
NACRES:
NA.77

Product Name

Interleukin-6 from rat, IL-6, recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture

biological source

rat

Quality Level

recombinant

expressed in E. coli

Assay

>97% (SDS-PAGE)

form

buffered aqueous solution

potency

0.01-0.06 ng/mL ED50

quality

endotoxin tested

mol wt

predicted mol wt ~22 kDa

packaging

pkg of 10 and 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.01EU/mg (LAL test)

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

rat ... Il6(24498)

Biochem/physiol Actions

Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-β-2 (IFN-β2), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.

Physical form

Supplied as a 0.2 μm filtered solution in 50 mM sodium acetate, pH 4, with BSA as a carrier protein.

Preparation Note

Recombinant, rat Interleukin-6 is produced from a DNA sequence encoding the mature rat IL-6 protein. The methionyl form of mature rat IL-6, a 188 amino acid protein, has a predicted molecular mass of approx. 22 kDa. Rat and mouse IL-6 share 93% amino acid sequence identity.

Analysis Note

The biological activity is measured in a cell proliferation assay using IL-6 dependent mouse plasmacytoma cell line, T1165.85.2.1.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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