I2406

IL-11 human

>97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

• Synonym(s): Interleukin-11 human, IL-11
• MDL number: MFCD00212208
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• Product Name: Interleukin-11 human, >97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture
• biological source: human
• Quality Level: 100
• recombinant: expressed in baculovirus infected Sf21 cells
• Assay: >97% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.06-0.24 ng/mL ED₅₀
• mol wt: 23 kDa
• packaging: pkg of 5 μg
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P20809
• storage temp.: −20°C
• Gene Information: human ... IL11(3589)

Description

General description

Interleukin-11 (IL-11) is synthesized by many mesenchymal origin cells like keratinocytes, synoviocytes, fibroblasts, bone marrow stromal cells, osteoblasts, and chondrocytes.

The gene IL-11 (interleukin 11) is mapped to human chromosome 19q13.4. It is a secreted protein.

Biochem/physiol Actions

Interleukin 11 (IL-11) is involved in promoting tissue fibrosis and regulates the function of chondrocytes, synoviocytes, and B cells. It is also involved in bone metabolism and tissue remodeling.

Interleukin-11, also called adipogenesis inhibitory factor (AGIF), is a pleotropic cytokine that acts on hematopoietic progenitor cells and stromal cells. Although recombinant human IL-11 has a predicted molecular mass of 19 kDa, it migrates as a 23 kDa band in SDS-PAGE. It is a functional homolog of IL-6, inducing the proliferation of certain plasmacytoma cell lines and acute phase protein secretion in the liver. IL-11 stimulates the production of erythrocytes, megakaryocytes, and T cell development of antibody producing B cells.

Physical form

Lyophilized from a 0.2 μm filtered solution of PBS and EDTA with BSA as a carrier protein.

Analysis Note

The biological activity is measured in a cell proliferation assay using T11, a subline of the IL-6-dependent murine plasmacytoma cell line T1165.85.2.1 that has been adapted to grow in IL-11.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves