I2411
Isocitrate Dehydrogenase 1 (NADP+) human
recombinant, expressed in Sf9 cells, ≥90% (SDS-PAGE)
Synonym(s):
IDH1, Isocitrate Dehydrogenase Cytoplasmic
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About This Item
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recombinant
expressed in Sf9 cells
Quality Level
Assay
≥90% (SDS-PAGE)
form
buffered aqueous glycerol solution
mol wt
47.6 kDa
concentration
≥0.30 mg/mL protein (Bradford)
NCBI accession no.
relevant disease(s)
cancer (lung)
shipped in
dry ice
storage temp.
−70°C
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General description
Human Isocitrate Dehydrogenase (IDH1), also known as oxalosuccinate decarboxylase, full length [amino acids 1-414 (end)] with C-terminal FLAG-tag, expressed in Sf9 cells via a baculovirus expression system.
Application
Human Isocitrate Dehydrogenase (IDH1) is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. It may be useful as a biomarker for diagnosis and prognosis prediction of non-small cell lung cancer .
Biochem/physiol Actions
Human Isocitrate Dehydrogenase (IDH1), also known as oxalosuccinate decarboxylase, is a full length amino acid with a C-terminal FLAG-tag and is expressed in Sf9 cells via a baculovirus expression system. IDH1 expression has been correlated with poor survival of non-small cell lung cancer. IDH1 promotes tumor growth .
Physical form
Solution in 45 mM Tris-HCl, 124 mM NaCl, 2.4 mM KCl, 90 μg/ml FLAG peptide, 3 mM DTT, and 10% glycerol at pH 8.0.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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