I3400

Interferon-γ-Inducible Protein-10 human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

• Synonym(s): CXCL10, IP-10
• MDL number: MFCD00241912
• UNSPSC Code: 51111800
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in E. coli
• Assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.015-0.075 μg/mL ED₅₀ (chemoattract mouse BaF3 cells transfected with hCXCR3); 0.03-0.18 μg/mL ED₅₀ (chemoattract T-Lymphocytes)
• mol wt: 8.7 kDa
• packaging: pkg of 10 μg
• impurities: endotoxin, tested
• UniProt accession no.: P01579
• storage temp.: −20°C
• Gene Information: human ... CXCL10(3627)

Description

General description

The gene for C-X-C motif chemokine ligand 10 (CXCL10), also known as interferon-γ-inducible protein-10 (IP-10), is mapped to human chromosome 4q21.1. IP-10 is secreted by monocytes, neutrophils, endothelial cells, keratinocytes, fibroblasts, mesenchymal cells, dendritic cells, astrocytes and hepatocytes. It is a polypeptide of 78 amino acids. Human IP-10 shows 67% amino acid homology to mouse CRG-2.

Biochem/physiol Actions

IP-10 (interferon-γ-inducible protein-10) protein is a proinflammatory chemokine which controls immune responses. It interacts with chemokine receptor 3 (CXCR3) and activates leukocytes including T cells, eosinophils, monocytes and natural killer cells. Mutations in IP-10 are associated with rheumatoid arthritis. In humans with chronic hepatitis C virus (HCV) infection or HCV/human immunodeficiency virus (HIV) co-infection, IP-10 is linked with obesity and insulin resistance.

IP-10, a chemoattractant for activated T lymphocytes, is a potent inhibitor of angiogenesis and displays thymus-dependent anti-tumor effects.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose containing 50 μg bovine serum albumin per 1 μg of cytokine.

Analysis Note

The biological activity is measured by its ability to chemoattract mouse BaF/3 cells transfected with hCXCR3.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable