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M7403

Sigma-Aldrich

MCDB 153 Medium

With trace elements, L-glutamine and 28mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

Molecular, Cellular, and Development Biology 153 medium

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10 μG
CA$637.00

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

CA$637.00

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Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
L-glutamine: 0.8772 g/L
glucose: 1.081 g/L (Dextro)
sodium pyruvate: 0.055 g/L
phenol red: 0.001242 g/L
HEPES: 6.6 g/L (25mM)

shipped in

ambient

storage temp.

2-8°C

General description

MCDB 153 media are modifications of Ham′s nutrient mixture F-12, designed for the growth of human keratinocytes, clonal growth of chicken embryo fibroblasts, and Chinese hamster ovary (CHO) cells using low levels of fetal bovine serum protein (FBSP), extensive trace elements or no serum protein.

Application

MCDB 153 Medium has been used as a component of melanoma isolation media (MIM) to culture human non-metastatic and metastatic melanoma cells.

Quantity

Formulated to contain 17.6 grams of powder per liter of medium.

Reconstitution

Supplement with 1.18 g/L sodium bicarbonate.

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This Item
M8537F8105M6770
Sigma-Aldrich

M7403

MCDB 153 Medium

MCDB 131 Medium With trace elements and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

M8537

MCDB 131 Medium

Sigma-Aldrich

F8105

MCDB 153 Medium

Sigma-Aldrich

M6770

MCDB 201 Medium

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

powder

form

powder

form

liquid

form

powder

components

NaHCO3: no
L-glutamine: 0.8772 g/L
glucose: 1.081 g/L (Dextro)
sodium pyruvate: 0.055 g/L
phenol red: 0.001242 g/L
HEPES: 6.6 g/L (25mM)

components

sodium pyruvate: 0.11 g/L
phenol red: 0.012 g/L
L-glutamine: 1.461 g/L
HEPES: no
glucose: 1.0 g/L (Dextro)

components

L-glutamine: no

components

HEPES: 7.149 g/L (30mM)
glucose: 1.441 g/L (Dextro)
phenol red: 0.001242 g/L
sodium pyruvate: 0.055 g/L
L-glutamine: 0.14615 g/L

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Quality Level

500

Quality Level

-

Quality Level

400

Quality Level

500

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCJ1063
    RNBJ5833
    SLCB9782
    RNBH1761
    SLBT9749

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