M9004

Malic Dehydrogenase from bovine heart

ammonium sulfate suspension, 2000-4000 units/mg protein (modified Warburg-Christian)

• Synonym(s): L-Malate:NAD⁺ oxidoreductase, MDH
• EC Number: 1.1.1.37 (BRENDA, IUBMB)
• EC Number: 232-622-5
• MDL number: MFCD00131567
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bovine heart
• Quality Level: 200
• form: ammonium sulfate suspension
• specific activity: 2000-4000 units/mg protein (modified Warburg-Christian)
• mol wt: 35 kDa
• impurities: ≤0.01% Glutamic-Oxalacetic Transaminase; ≤0.01% Glutamic-Pyruvic Transaminase
• storage temp.: 2-8°C

Description

General description

Malic Dehydrogenase from bovine heart contains a histidine residue at the NAD-binding active site which is critical for activity. When this histidine is mutated a loss in activity is observed.

Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.

Application

Malic dehydrogenase has been used in a study to assess a flow injection system for on-line monitoring of fumaric acid in biological processes. It has also been used in a study to investigate a root-knot nematode parasitizing peanut in Texas.

Unit Definition

One unit will convert 1.0 μmole of oxalacetate and β-NADH to _L-malate and β-NAD per min at pH 7.5 at 25 °C.

Physical form

Suspension in 3 M (NH₄)₂SO₄ - 0.01 M KH₂PO₄ solution, pH 7.3

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves