Skip to Content
Merck

Key Documents

View All Documentation

Skip To

M9943

Sigma-Aldrich

10X MTP™ Taq Buffer

10X buffer, free of DNA contaminants; use with MTP Taq DNA Polymerase

Sign Into View Organizational & Contract Pricing

About This Item

UNSPSC Code:
41106306
NACRES:
NA.52
Pricing and availability is not currently available.
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Quality Level

200

form

liquid

color

colorless

shipped in

wet ice

storage temp.

−20°C

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
D7442B5926P2317
10X MTP™ Taq Buffer 10X buffer, free of DNA contaminants; use with MTP Taq DNA Polymerase

M9943

10X MTP™ Taq Buffer

MTP™ Taq DNA Polymerase Taq DNA Polymerase, free of DNA contaminants

D7442

MTP™ Taq DNA Polymerase

10× REDTaq® PCR Reaction Buffer To be used with REDTaq® DNA Polymerase

B5926

10× REDTaq® PCR Reaction Buffer

10X PCR Buffer without MgCl2 Optimized for routine PCR without MgCl2

P2317

10X PCR Buffer without MgCl2

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

form

liquid

form

liquid

form

liquid

form

liquid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

color

colorless

color

colorless

color

colorless

color

colorless

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

wet ice

General description

DNA contaminants can be introduced into PCR through a number of reagents. To minimize the risk of contaminant DNA during PCR, we offer 10x MTP Taq buffer to be used with MTP Taq DNA Polymerase (Product No. D7442). Each lot of MTP Taq buffer undergoes strict quality control testing to ensure the absence of contaminating DNA. To prevent false positive PCR results, only DNA-free reagents should be used in PCR reactions with MTP Taq DNA polymerase.

Application

10X MTP™Taq Buffer has been used:

  • for the amplification of bacterial 16s rRNA genes from purified DNA [1]
  • to amplify archaeal 16s rRNA [2]
  • along with MTP Taq DNA polymerase (D7442) to amplify specific bacterial 16s rRNA genes from purified DNA [3]

Legal Information

MTP is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCC6997
    SLCB6182
    SLBW8392
    SLBW0584
    SLBV3089

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Search for a COA

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Florie Maillard et al.
    Cells, 8(1) (2019-01-13)
    Crohn's disease is characterized by abnormal ileal colonization by adherent-invasive E. coli (AIEC) and expansion of mesenteric adipose tissue. This study assessed the preventive effect of spontaneous physical activity (PA) on the gut-adipose tissue in a mouse model that mimics
    Risk assessment models and contamination management: implications for broad-range ribosomal DNA PCR as a diagnostic tool in medical bacteriology.
    B Cherie Millar et al.
    Journal of clinical microbiology, 40(5), 1575-1580 (2002-05-01)
    Florie Maillard et al.
    Cells, 8(1) (2019-01-13)
    Crohn's disease is characterized by abnormal ileal colonization by adherent-invasive E. coli (AIEC) and expansion of mesenteric adipose tissue. This study assessed the preventive effect of spontaneous physical activity (PA) on the gut-adipose tissue in a mouse model that mimics
    C E Corless et al.
    Journal of clinical microbiology, 38(5), 1747-1752 (2000-05-02)
    A set of universal oligonucleotide primers specific for the conserved regions of the eubacterial 16S rRNA gene was designed for use with the real-time PCR Applied Biosystems 7700 (TaqMan) system. During the development of this PCR, problems were noted with

    Protocols

    Low Contaminant Amplification of DNA Using MTP™ Taq DNA Polymerase

    Method for bacterial genome analysis and detection of pathogens. Minimize false positive PCRs through lab design and reagents tested for use in bacterial PCR applications.

    Method for PCR amplification of Bacterial DNA with low contamination using MTP™ Taq DNA Polymerase (D7442)

    Method for PCR amplification of Bacterial DNA with low contamination using MTP™ Taq DNA Polymerase (D7442)

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service