N7271

α(2→3) Neuraminidase from Streptococcus pneumoniae

buffered aqueous solution

• Synonym(s): Acyl-neuraminyl Hydrolase, Acylneuraminyl hydrolase, Receptor-destroying enzyme, Sialidase
• CAS Number: 9001-67-6
• EC Number: 3.2.1.18 (BRENDA, IUBMB)
• MDL number: MFCD01092203
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• biological source: Streptococcus pneumoniae
• Quality Level: 100
• form: buffered aqueous solution
• concentration: ≥5 units/mL
• foreign activity: β-Galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, and proteases, none detected
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: Streptococcus pneumoniae R6 ... nanB(933504)

Description

Biochem/physiol Actions

Releases α(2→3)-linked N-acetylneuraminic acid from complex oligosaccharides.

Packaging

Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).

Unit Definition

One unit will hydrolyze 1μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37 °C.

Physical form

Solution in 50 mM sodium phosphate, pH 7.5

Preparation Note

Expressed in glycosidase-free hosts.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves