N7660
4-Nitrophenol
chromogenic, liquid
Synonym(s):
p-Nitrophenol solution
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About This Item
Empirical Formula (Hill Notation):
C6H5NO3
CAS Number:
Molecular Weight:
139.11
MDL number:
UNSPSC Code:
12352204
eCl@ss:
39032018
PubChem Substance ID:
NACRES:
NA.28
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Product Name
4-Nitrophenol solution, 10 mM
form
liquid
Quality Level
concentration
10 mM
storage temp.
2-8°C
SMILES string
Oc1ccc(cc1)[N+]([O-])=O
InChI
1S/C6H5NO3/c8-6-3-1-5(2-4-6)7(9)10/h1-4,8H
InChI key
BTJIUGUIPKRLHP-UHFFFAOYSA-N
Gene Information
human ... UGT1A4(54657)
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General description
4-Nitrophenol is a phenolic compound present as a chemical intermediate and breakdown product during the production of organophosphorus insecticide derivatives of parathion. It is an intermediate during the synthesis of paracetamol. 4-Nitrophenol is a chromogenic catalytic by product of the synthetic substrate 4-nitrophenyl phosphate. It is majorly used as a reference standard in alkaline phosphatase assay. 4-Nitrophenol is nonbiodegradable and its derivatives are used for herbicide and fungicide production.
Application
4-Nitrophenol solution has been used:
- in the generation of standard curve for quantifying alkaline phosphatase activity of mesenchymal stem cells
- bone marrow stromal cells (BMSCs)
- for quantifying β-hexosaminidase activity in HeLa cells
- as a standard for calorimetric determination of alkaline phosphatase activity at 405 nm
- for assessing the β-hexosamindase activity
Preparation Note
Prepared as a 1.39 mg/ml solution suitable for use as a calibrator in alkaline phosphatase assays (such as Bessey-Lowry-Brock method) using p-nitrophenol phosphate as a substrate.
Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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