NUC201
Nuclei Isolation Kit: Nuclei PURE Prep
sufficient for 15 nuclei preparations (~1-10×107 cells or 1g of tissue per preparation)
Synonym(s):
Sucrose centrifugation nuclei isolation
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.32
Recommended Products
usage
sufficient for 15 nuclei preparations (~1-10×107 cells or 1g of tissue per preparation)
Quality Level
packaging
pkg of 1 kit
storage condition
dry at room temperature
application(s)
cell analysis
foreign activity
nuclease and protease, free
shipped in
wet ice
storage temp.
2-8°C
Application
For preparation of pure nuclei and fragile nuclei from cell lines and solid tissues.
Biochem/physiol Actions
The protocol incorporates centrifugation through a dense sucrose cushion to protect nuclei and strip away cytoplasmic contaminants. High yield has been obtained from common cell lines (Jurkat, HFN7.1, COS7, HEK293 and MDCK) and tissues (spleen and liver). These preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones, and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.
The protocol incorporates centrifugation through a dense sucrose cushion to protect nuclei and strip away cytoplasmic contaminants. The sucrose concentration that is suitable for a particular cell type is determined empirically by the user. The sucrose concentrate and sucrose cushion buffer give the user flexibility to modify the density of the sucrose cushion as appropriate. High yield has been obtained from common cell lines (Jurkat, HFN7.1, COS7, HEK293 and MDCK) and tissues (spleen and liver). These preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones, and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.
Kit Components Only
Product No.
Description
- Nuclei PURE Lysis Buffer 180 mL
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Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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