Skip to Content
Merck
All Photos(1)

Key Documents

P1588

Sigma-Aldrich

Placenta Growth Factor human

≥95% (SDS-PAGE), recombinant, expressed in E. coli, powder, suitable for cell culture

Synonym(s):

PGF, PlGF

Sign Into View Organizational & Contract Pricing


About This Item

MDL number:
UNSPSC Code:
12352202
NACRES:
NA.77

Product Name

Placenta Growth Factor human, PlGF, recombinant, expressed in E. coli, powder, suitable for cell culture

biological source

human

Quality Level

recombinant

expressed in E. coli

Assay

≥95% (SDS-PAGE)

form

powder

quality

endotoxin tested

mol wt

29 kDa

packaging

pkg of 10 and 50 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

<1.0 EU/μg

shipped in

dry ice

storage temp.

−70°C

Biochem/physiol Actions

Placenta growth factor (PlGF) is expressed in the placenta and somewhat less in the heart, lung and thyroid gland. Placentally expressed PlGF may act as an autocrine on trophoblasts, which express both PlGF and its receptor (VEGFR-1). Since these cells also make VEGF-A, natural heterodimers (PlGF/VEGF-A) have also been detected. Two alternatively spliced isoforms of PlGF have been identified.

Physical form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 50 μg bovine serum albumin per 1 μg of cytokine.

Preparation Note

Recombinant human PlGF is lyophilized from a 0.2 micron filtered solution of 30% CH3CN and 0.1% TFA (pH 7.4) containing 50 microg of bovine serum albumin per 1 microg of cytokine.

Reconstitution

Reconstitute with sterile PBS containing at least 0.1% HSA or BSA. The rhP/GF concentration should be no less than 10 μg/mL.

Analysis Note

The biological activity is measured by the ability to bind rhFlt-1/Fc in a functional ELISA. Immobilized rhFlt-1/Fc at 2 μg/mL (100 μL/well) can bind rhP/GF with a linear range at 0.1 - 5 ng/mL.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service