P2143

Protease from Aspergillus saitoi

Type XIII, ≥0.6 unit/mg solid

• Synonym(s): Molsin
• CAS Number: 9025-49-4
• EC Number: 3.4.23.18 (BRENDA, IUBMB)
• EC Number: 232-796-2
• MDL number: MFCD00132092
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• NACRES: NA.54

Properties

• biological source: Aspergillus sp. (Aspergillus saitoi)
• Quality Level: 200
• type: Type XIII
• form: solid
• specific activity: ≥0.6 unit/mg solid
• foreign activity: alkaline protease, essentially free
• storage temp.: −20°C

Description

Application

Protease from Aspergillus saitoi has been used in a study to assess sequence coverage and resolution in hydrogen exchange of large proteins. It has also been used in a study to investigate the conversion of soybean curd isoflavone glycosides to their aglycones through β-glucosidase..

Biochem/physiol Actions

Protease from Aspergillus saitoi was shown to also function as a β-glucosidase.

Unit Definition

One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 2.8 at 37 °C (color by Folin-Ciocalteu reagent).

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H315,H319,H334,H335
• Precautionary Statements: P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable