P3134

Phosphodiesterase I from Crotalus adamanteus venom

Type VI, crude dried venom

• Synonym(s): 5′-Exonuclease, Oligonucleate 5′-nucleotidohydrolase
• CAS Number: 9025-82-5
• EC Number: 3.1.4.1 (BRENDA, IUBMB)
• EC Number: 232-806-5
• MDL number: MFCD00131883
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• type: Type VI
• Quality Level: 200
• form: crude dried venom
• storage temp.: −20°C

Description

General description

Research area: Cell signaling. PhosphodiesteraseI (PDEI) exists in three isoforms PDE1A, PDE1B, and PDE1C(2) are localized to the central nervous system, cardiovascular, and other organs.[1]

Application

Phosphodiesterase I from Crotalus adamanteus venom has been used:

• for cleaving the 3′-5′ internucleotide bonds in the linear chains of the RNA oligomers[2]
• as a model for the hydrolysis of prodrugs in phosphodiesterase assay[3]
• as a component in the nucleotide cleavage buffer [4]

Biochem/physiol Actions

Phosphodiesterase I breaks phosphodiester bonds and catalyzes the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is released from eucaryotic plasma membranes by phosphatidylinositol-specific phospholipase C. PhosphodiesteraseI (PDEI) can hydrolyze 3′,5′-cyclic adenosine monophosphate (cAMP) and3′,5′-cyclic guanosine monophosphate (cGMP). It binds to Ca²⁺/calmodulin(CaM) that lead to the stimulation of its catalytic activity.PDEIacts as a biotherapeutic for the treatment of cancer, heart diseases,pulmonary, metabolic, neurocognitive, renal, endocrine abnormalities, andneurological disorders.[1]

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable