P4553

6-Phosphogluconic Dehydrogenase from yeast

lyophilized powder, ≥3.0 units/mg solid

• Synonym(s): 6-Phospho-D-gluconate:NADP⁺ 2-oxidoreductase (decarboxylating), 6-Phosphogluconic Dehydrogenase from yeast
• CAS Number: 9073-95-4
• EC Number: 1.1.1.44 (BRENDA, IUBMB)
• MDL number: MFCD00130317
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: yeast
• Quality Level: 200
• form: lyophilized powder
• specific activity: ≥3.0 units/mg solid
• mol wt: 52 kDa
• storage temp.: 2-8°C

Description

General description

6-Phosphogluconic Dehydrogenase from yeast comprises of a NADP⁺ binding domain, α helical hydrophobic interaction domain and a C-terminal domain. It exists as dimer and the C-terminal region is crucial for the substrate binding and release.

Application

6-Phosphogluconic Dehydrogenase from yeast has been used in hexokinase (HK) and xylose reductase (XR) activities in cell lysate.

Biochem/physiol Actions

Enzyme activity is determined by measuring the reduction of NAD+ or NADP+. High levels of NADPH are believed to inhibit the enzyme, while 6-phosphogluconate acts to activate the enzyme.

Unit Definition

One unit will oxidize 1.0 μmole of 6-phospho-D-gluconate to D-ribulose 5-phosphate and CO₂ per min at pH 7.4 at 37 °C in the presence of NADP⁺.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves