P7173

Pyruvate Carboxylase from bovine liver

buffered aqueous glycerol solution, 5-25 units/mg protein (BCA)

• Synonym(s): Pyruvate:CO₂ ligase (ADP-forming)
• CAS Number: 9014-19-1
• EC Number: 6.4.1.1 (BRENDA, IUBMB)
• MDL number: MFCD00132143
• UNSPSC Code: 12352204
• NACRES: NA.26

Properties

• form: buffered aqueous glycerol solution
• Quality Level: 200
• specific activity: 5-25 units/mg protein (BCA)
• concentration: ≥0.5 mg/mL
• foreign activity: lactic dehydrogenase ≤0.5%
• storage temp.: −20°C

Description

Application

Pyruvate is critical for gluconeogenesis, lipogenesis, glyceroneogenesis, neurotransmitter biosynthesis and glucose-induced insulin, and is used to study these processes.

The enzyme from Sigma has been used as a positive control during the assay of pyruvate carboxylase activity in cell-free extracts of Corynebacterium glutamicum.

Biochem/physiol Actions

Pyruvate carboxylase catalyzes the carboxylation of pyruvate to oxaloacetate. Pyruvate carboxylase is a mitochondrial protein that has a biotin prosthetic group that requiries magnesium or manganese and acetyl CoA.

Unit Definition

One unit will convert 1.0 μmole of pyruvate and CO₂ to oxalacetate per min at pH 7.8 at 30 °C.

Physical form

Solution in 50% glycerol containing 0.05 M Tris-HCl, pH 7.4, 2 mM magnesium acetate and 1 mM EDTA.

Preparation Note

Affinity purified

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable