P7275

Pyrophosphate reagent

spectrophotometric assay

• Synonym(s): Pyrophosphate detection reagent
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• Quality Level: 200
• form: powder
• usage: vial sufficient for ≤4 assays
• storage temp.: −20°C

Description

Application

The product has been used for the measurement of pyrophosphate content of plant tissues. It has also been used as a rapid, routine check of pyrophosphate content by monitoring NADH oxidation in Solanum tuberosum tuber extracts.

Biochem/physiol Actions

The pyrophosphate detection kit couples the production of pyrophosphate to the consumption of NADH.

Packaging

A technical bulletin is included.

Components

ComponentConcentration
Imidazole × HCl, pH 7.4 45 mM
Citrate 5 mM
EDTA 0.10 mM
Mg²⁺, Mn²⁺, Co²⁺ 2 mM, 0.2 mM, 0.02 mM
β-NADH 0.8 mM
F-6-P 12 mM
Bovine Serum Albumin 5 mg/mL
Sugar Stabilizer 5 mg/mL
PPi-PFK 0.5 units/mL
Aldolase 7.5 units/mL
GDH 5 units/mL
TPI 50 units/mL

Quality

A coupled enzyme system containing fructose-6-phosphate kinase (pyrophosphate-dependent), aldolase, triosephosphate isomerase, and α-glycerophosphate dehydrogenase.

Principle

Two moles of NADH are oxidized to NAD⁺ per mole of pyrophosphate consumed. The reaction is monitored spectrophotometrically at 340 nm.
The reagent can be used to detect 10-100 nmoles of pyrophosphate.

Preparation Note

Dissolve the contents of one vial of Pyrophosphate Reagent in 4.0 mL of water.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves