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Key Documents

P7777

Sigma-Aldrich

Poly(ethylene glycol)

Hybri-Max, mol wt 1,300-1,600, waxy solid, BioReagent, suitable for hybridoma

Synonym(s):

PEG

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About This Item

Linear Formula:
H(OCH2CH2)nOH
CAS Number:
MDL number:
UNSPSC Code:
12352104
PubChem Substance ID:
NACRES:
NA.75

biological source

synthetic (organic)

Quality Level

grade

Hybri-Max

sterility

autoclaved

product line

BioReagent

form

waxy solid

mol wt

1,300-1,600

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

viscosity

25-35 cP (210 °F)

mp

40-50 °C

solubility

water: soluble (5 mL water/vial at 45 °C)

SMILES string

C(CO)O

InChI

1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2

InChI key

LYCAIKOWRPUZTN-UHFFFAOYSA-N

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Application

Recommended for cell fusion.

Reconstitution

Melt the PEG at 45 °C or higher in a waterbath. Dilute to 30-50% using sterile, pre-warmed serum-free medium (e.g., for a 50% solution add 5 mL to the 5 g vial of PEG). The pH of the solution should be slightly alkaline (pink, not orange or purple). Adjustment of the pH with sterile NaOH may be required. Store solution below 0 °C. Prolonged storage or repeated freezing and thawing is not recommended.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

281.5 °F - closed cup

Flash Point(C)

138.6 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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