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PC0100

Sigma-Aldrich

Protease Colorimetric Detection Kit

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100 μG
CA$442.00

About This Item

UNSPSC Code:
12352202

CA$442.00

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storage temp.

2-8°C

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Corning® CoolRack® M30-PF, capacity 30 x 1.5 mL microfuge tubes, tapered wells for conical tubes, gray

CLS432048

Corning® CoolRack®

Corning® CoolRack® 500 ul M30-PF, capacity 30 x 500 ul microfuge tubes, tapered wells for conical tubes, gray

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Corning® CoolRack® M30, capacity 30 x 1.5 or 2 mL microfuge tubes, gray

CLS432041

Corning® CoolRack®

Corning® CoolRack® M30, capacity 30 x 1.5 or 2 mL microfuge tubes, green

CLS432042

Corning® CoolRack®

feature

Holds 30 x 1.5 mL tubes

feature

Holds 30 x 0.5 mL tubes

feature

wells 30, Holds 30 x 1.5-2 mL tubes

feature

wells 31, Holds 30 x 1.5-2 mL tubes

manufacturer/tradename

Corning 432048

manufacturer/tradename

Corning 432046

manufacturer/tradename

Corning 432041

manufacturer/tradename

Corning 432042

material

foamed PE

material

foamed PE

material

foamed PE

material

foamed PE

packaging

pkg of 1

packaging

pkg of 1

packaging

pkg of 1

packaging

pkg of 1

technique(s)

cryopreservation: suitable

technique(s)

cryopreservation: suitable

technique(s)

cryopreservation: suitable

technique(s)

cryopreservation: suitable

color

gray

color

gray

color

gray

color

green

Application

The Protease Colorimetric Detection Kit is designed for the accurate quantitation of protease activity. The method is based on the cleavage of casein into trichloroacetic acid-soluble peptides containing tyrosine and tryptophan residues. These peptides react with Folin-Ciocalteu reagent to give a characteristic blue color that can be measured at 660 nm.

Suitability

Detection limit: 0.1 unit/ml (approx. 2 μg) trypsin with a 10 minute incubation time.

Linkage

For greater sensitivity or for detection of trace protease activity, the Protease Fluorescent Detection kit (PF0100) is recommended.

Quantity

Supplied with sufficient reagents to perform 200 one mL assays.

Analysis Note

This kit has been tested for detection of all four proteolytic classes (serine, apartic, cysteine and metalloproteinases).

Kit Components Only

Product No.
Description
  • Protease detection substrate (casein) 20 x 10
  • Folin & Ciocalteu's phenol reagent 10 mL
  • Sodium carbonate solution 125 mL
  • Enzyme diluent 125 mL
  • Tyrosine standard solution 1 mL
  • Protease from Bacillus polymyxa 1 g
  • Trichloroacetic acid (TCA) solution 5 mL

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Description
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    094K6064

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