R1028

Restorase^® DNA Polymerase with 10× Reaction Buffer

Enzyme blend for PCR amplification of damaged DNA

• UNSPSC Code: 12352202
• NACRES: NA.55

Properties

• Quality Level: 200
• form: liquid
• usage: sufficient for 50 reactions
• feature: Long & Accurate PCR; dNTPs included: no; hotstart: no
• concentration: 2.5 units/μL
• technique(s): PCR: suitable
• color: colorless
• input: purified DNA
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Restorase DNA Polymerase with 10× Reaction Buffer combines Sigma′s long and accurate enzyme technology with a small amount of DNA repair enzyme. The optimized blend will initiate the repair and further amplification of damaged DNA templates greater than 800 bp. Restorase has also been shown to increase yield on undamaged DNA templates.

Application

Restorase^® DNA Polymerase with 10× Reaction Buffer has been used in DNA repair.

Biochem/physiol Actions

DNA templates are often damaged on exposure to heat, acids, alkylating agents, and light. These damaged templates result in inefficient or failed DNA amplification by polymerase chain reaction (PCR). Restorase^® DNA Polymerase repairs the damaged sites on the DNA templates and initiates subsequent template copying. Restorase treatment of the DNA depends on the level of template damage.

Features and Benefits

• Reliable amplification of damaged DNA
• When thermostable polymerases fail, this enzyme amplifies the sequence efficiently
• Efficient amplification of sequences in multiplex reactions
• Increased yield and amplicon specificity
• Amplifies a broad spectrum of amplicon sizes varying from 200 bp to 20 kb
• Can repair 3′ bungs, nicks, and abasic sites

• Reliable amplification of damaged DNA
• Increase Yield
• Can repair 3′ bungs, nicks, and abasic sites

Packaging

The enzyme is provided with an optimized 10× reaction buffer provided as 1 vial/250 units.

Other Notes

Learn more about our offering of specialty enzymes at www.sigma-aldrich.com/specialtyenzymes.

View more detailed information on Restorase DNA Polymerase at www.sigma-aldrich.com/restorase.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Restorase is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Hazard Statements: H412
• Precautionary Statements: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class Code: 10 - Combustible liquids