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SAE0097

Sigma-Aldrich

D-2-Hydroxyglutarate Dehydrogenase (D2HGDH) from Acidaminococcus fermentans

recombinant, expressed in E. coli, aqueous solution

Synonym(s):

D2HGDH, HGDH, L-2-hydroxyglutarate dehydrogenase

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About This Item

EC Number:
1.1. 99.2
UNSPSC Code:
12352202
NACRES:
NA.54

recombinant

expressed in E. coli

Assay

≥95% (SDS-PAGE)

form

aqueous solution

specific activity

≥1000 units/mg protein

UniProt accession no.

D2RJU7

shipped in

wet ice

storage temp.

−20°C

General description

D-2-Hydroxyglutarate Dehydrogenase (D2HGDH) is a member of the D-2-hydroxyacid NAD+ dependent dehydrogenase family of proteins. D2HGDH catalyzes the conversion of α-ketoglutarate (α--KG) to D-2-hydroxyglutarate (D2HG), coupled to the oxidation of NADH to NAD+ .

The crystal structure of D2HGDH from Acidaminococcus fermentans has been reported. D2HGDH from Acidaminococcus fermentans has been used in several enzymatic assays, such as:
  • A continuous spectrophotometric assay to measure the activity of aminotransferases, based on the transamination of a keto compound and L-glutamate, which yields a corresponding amino compound and 2-oxoglutarate.
  • Determination of D2HG levels in biological fluids such as serum, urine, cell culture supernatants, and cell or tissue lysates.
  • A coupled assay system to measure branched-chain amino acid aminotransferase activity.

Unit Definition

One unit of enzyme oxidizes 1 μmole of NADH to NAD+ coupled to the reduction of α-ketoglutarate to (D)-2-hydroxyglutarate per minute at 37°C at pH 8.0.

Preparation Note

This recombinant D2HGDH product is supplied as an aqueous solution in 20 mM Trizma® buffer, pH 7.5, with 150 mM NaCl, and 10% glycerol.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
118M4178V
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