SML4060

NIAD-4

≥98% (HPLC)

• Synonym(s): 2-((5′-(4-Hydroxyphenyl)-2,2′-bithiophen-5-yl)methylene)malononitrile, 2-[[5′-(4-Hydroxyphenyl)[2,2′-bithiophen]-5-yl]methylene]propanedinitrile, 2-[[5-[5-(4-Hydroxyphenyl)thiophen-2-yl]thiophen-2-yl]methylidene]propanedinitrile, 5’-(4-Hydroxyphenyl)-5-(2,2-dicyanoethenyl)-2,2’-bithiophene, NIAD 4, NIAD4
• Empirical Formula (Hill Notation): C₁₈H₁₀N₂OS₂
• CAS Number: 868592-56-7
• Molecular Weight: 334.41
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: ≥98% (HPLC)
• form: powder
• color: , Red to very dark red-brown
• solubility: DMSO: 2 mg/mL, clear
• storage temp.: 2-8°C

Description

Biochem/physiol Actions

Blood-brain barrier (BBB)-permeable, far-red (near-IR) fluorescent contrast agent for staining of amyloid plaques with high specificity in situ and in vivo.

NIAD-4 is a blood-brain barrier (BBB)-permeable, far-red (near-IR) fluorescent contrast agent for staining of amyloid plaques with high specificity in situ (10 μM in DMSO/propylene glycol; APP mice coronal section) and in vivo (2 mg/kg APP mice i.v.). Due to its push-pull structure design and a greatly enhanced thioflavin T (ThT) site affinity (Ki = 10 nM/NIAD-4 vs 580 nM/ThT), NIAD-4 exhibits significantly red-shifted (~70 nm) absorption and a 1.3-fold increase in extinction coefficient upon binding aggregated amyloid-beta 1-40 (4.1 μM NIAD-4 & 10 μM amyloid-β in PBS). Ex 450-488 nm, Em 550-650 nm.