Skip to Content
Merck

Key Documents

View All Documentation

Skip To

SRE0006

Sigma-Aldrich

Thymidine Phosphorylase, recombinant from Escherichia coli

recombinant, expressed in E. coli, Suitable for manufacturing of diagnostic kits and reagents, buffered aqueous solution, ≥500 units/mL

Synonym(s):

Gliostatins, PD-ECGF, Thymidine:orthophosphate deoxy-D-ribosyltransferase

Sign Into View Organizational & Contract Pricing

Select a Size

100 G
CA$116.00
500 G
CA$463.00
1 KG
CA$684.00

CA$116.00

Please contact Customer Service for Availability
Request a Bulk Order

Select a Size

Change View
100 G
CA$116.00
500 G
CA$463.00
1 KG
CA$684.00

About This Item

CAS Number:
9030-23-3
EC Number:
2.4.2.4 (BRENDA, IUBMB)
MDL number:
MFCD00132426
UNSPSC Code:
12352204
NACRES:
NA.54

CA$116.00

Please contact Customer Service for Availability
Request a Bulk Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

recombinant

expressed in E. coli

Quality Level

400

form

buffered aqueous solution

concentration

≥500 units/mL

technique(s)

inhibition assay: suitable

color

colorless to yellow

solubility

soluble
water: soluble

NCBI accession no.

NP_418799.1

UniProt accession no.

P07650

application(s)

diagnostic assay manufacturing

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
EHU106121EHU072971EHU133471
MISSION® esiRNA targeting human MAPK9

EHU080681

MISSION® esiRNA

MISSION® esiRNA targeting human MAPK6

EHU106121

MISSION® esiRNA

MISSION® esiRNA targeting human MAPK8

EHU072971

MISSION® esiRNA

MISSION® esiRNA targeting human MAPK7

EHU133471

MISSION® esiRNA

Gene Information

human ... MAPK9(5601), MAPK9(5601)

Gene Information

human ... MAPK6(5597), MAPK6(5597)

Gene Information

human ... MAPK8(5599), MAPK8(5599)

Gene Information

human ... MAPK7(5598), MAPK7(5598)

Ensembl | human accession no.

ENSG00000050748

Ensembl | human accession no.

ENSG00000069956

Ensembl | human accession no.

ENSG00000107643

Ensembl | human accession no.

ENSG00000166484

product line

MISSION®

product line

MISSION®

product line

MISSION®

product line

MISSION®

esiRNA cDNA target sequence

GCAAACCTGTCAGCATTGAAGGAACTCTCACCTCCGTGGGCCTGAAATGCTTGGGAGTTGATGGAACCAAATAGAAAAACTCCATGTTCTGCATGTAAGAAACACAATGCCTTGCCCTACTCAGACCTGATAGGATTGCCTGCTTAGATGATAAAATGAGGCAGAATATGTCTGAAGAAAAAAATTGCAAGCCACACTTCTAGAGATTTTGTTCAAGATCATTTCAGGTGAGCAGTTAGAGTAGGTGAATTTGTTTCAAATTGTACTAGTGACAGTTTCTCATCATCTGTAACTGTTGAGATGTATGTGCATGTGACCACAAATGCTTGCTTGGACTTGCCCATCTAGCACTTTGGAAATCAGTATTTAAATGCCAAATAATCTTCCAGGTAGTGCTGCTTCTG

esiRNA cDNA target sequence

GTCCCAGTGGAAGCCAATTAACAGACGATGTGGGCTCTCTTACGGAACTGAACAGTGTTTACATTGTTCAGGAGTACATGGAGACAGACTTGGCTAATGTGCTGGAGCAGGGCCCTTTACTGGAAGAGCATGCCAGGCTTTTCATGTATCAGCTGCTACGGGGGCTCAAGTATATTCACTCTGCAAATGTACTGCACAGAGATCTCAAACCAGCTAATCTTTTCATTAATACGGAAGACTTGGTGCTGAAGATAGGTGACTTTGGTCTTGCACGGATCATGGATCCTCATTATTCCCATAAGGGTCATCTTTCTGAAGGATTGGTTACTAAATGGTACAGATCTCCACGTCTTTTACTTTCTCCTAATAATTATACTAAAGCCATTGACATGTGGGCTGCAGGCTGCATCTTTGCTGAAATGCTGACTGG

esiRNA cDNA target sequence

AAGCTAAGCCGACCATTTCAGAATCAGACTCATGCCAAGCGGGCCTACAGAGAGCTAGTTCTTATGAAATGTGTTAATCACAAAAATATAATTGGCCTTTTGAATGTTTTCACACCACAGAAATCCCTAGAAGAATTTCAAGATGTTTACATAGTCATGGAGCTCATGGATGCAAATCTTTGCCAAGTGATTCAGATGGAGCTAGATCATGAAAGAATGTCCTACCTTCTCTATCAGATGCTGTGTGGAATCAAGCACCTTCATTCTGCTGGAATTATTCATCGGGACTTAAAGCCCAGTAATATAGTAGTAAAATCTGATTGCACTTTGAAGATTCTTGACTTCGGTCTGGCCAGGACTGCAGGAACGAGTTTTATGATGACGCCTTATGTAGTGACTCGCTACTACAGAGCACCCGAGGTCATCCTTGGCATGGGCTACAAGGAAAAC

esiRNA cDNA target sequence

CCTTGTGACCCAGCAGCTATCTAAGTCACAGGTGGAGGACCCCCTGCCCCCTGTGTTCTCAGGCACACCAAAGGGCAGTGGGGCTGGCTACGGTGTTGGCTTTGACCTGGAGGAATTCTTAAACCAGTCTTTCGACATGGGCGTGGCTGATGGGCCACAGGATGGCCAGGCAGATTCAGCCTCTCTCTCAGCCTCCCTGCTTGCTGACTGGCTCGAAGGCCATGGCATGAACCCTGCCGATATTGAGTCCCTGCAGCGTGAGATCCAGATGGACTCCCCAATGCTGCTGGCTGACCTGCCTGACCTCCAGGACCCCTGAGGCCCCCAGCCTGTGCCTTGCTGCCACAGTAGACCTAGTTCCAGGATCCATGGGAGCATTCTCAAAGGCTTTAGCCCTGGACCCAGCAGGTGAGGCTCGGCTTGGATTATTCTGCAGGTTCATCTCAGACCCACC

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

Research area: CELL SIGNALING

The E. coli thymidine phosphorylase shares 40% sequence homology with the human sequence, which is identical to the angiogenic agent platelet-derived endothelial growth factor. The purified E. coli enzyme has been shown to stimulate blood vessel growth in chick chorioallantoic membrane assays.

Application

Thymidine phosphorylase has been used:
  • in a study to evaluate biomarkers for advanced breast cancer patients treated with capecitabine-based first-line chemotherapy.[1]
  • in a study to investigate implications for the clinical efficacy of nucleoside analogues.[2]

Biochem/physiol Actions

An enzyme that catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the pyrimidine nucleoside salvage pathway.
An enzyme that catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the pyrimidine nucleoside salvage pathway. This pathway allows pyrimidine bases to be recycled for nucleotide biosynthesis, while the pentose 1-phosphates are converted to intermediates of the pentose phosphate shunt and glycolysis. The E. coli thymidine phosphorylase shares 40% sequence homology with the human sequence, which has been found to be identical to the angiogenic agent platelet-derived endothelial growth factor. The purified E. coli enzyme has been shown to stimulate blood vessel growth in chick chorioallantoic membrane assays.
Thymidine phosphorylase catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the thymidine salvage pathway [3] and pyrimidine nucleoside salvage pathway.[4] This pathway allows pyrimidine bases to be recycled for nucleotide biosynthesis, while the pentose 1-phosphates are converted to intermediates of the pentose phosphate shunt and glycolysis.[5] The enzyme inhibits apoptosis and induces angiogenesis thereby promoting tumor growth and metastatic process. [4] Moreover, thymidine phosphorylase inhibits vascular smooth muscle cell proliferation.

Unit Definition

One unit will convert 1.0 μmole each of thymidine and phosphate to thymine and 2-deoxyribose 1-phosphate per min at pH 7.4 at 25°C.

Preparation Note

Cloned from E. coli and produced in overexpressing E. coli

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    50180502
    50180515
    50180514
    SLBJ9704V
    SLBR8642V

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Search for a COA

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Leon Caly et al.
    Biochemical and biophysical research communications, 483(1), 64-68 (2017-01-08)
    Respiratory syncytial virus (RSV) is a major cause of respiratory infections in infants and the elderly, leading to more deaths than influenza each year worldwide. With no RSV antiviral or efficacious vaccine currently available, improved understanding of the host-RSV interaction
    Takouhie Mgrditchian et al.
    Proceedings of the National Academy of Sciences of the United States of America, 114(44), E9271-E9279 (2017-10-29)
    While blocking tumor growth by targeting autophagy is well established, its role on the infiltration of natural killer (NK) cells into tumors remains unknown. Here, we investigate the impact of targeting autophagy gene Beclin1 (BECN1) on the infiltration of NK
    Ting Li et al.
    Acta pharmacologica Sinica, 36(12), 1503-1513 (2015-11-26)
    Platycodin D, the main saponin isolated from Chinese herb Platycodonis Radix, exhibits anticancer activities against various cancer cell lines. Here we evaluated its anticancer action against human hepatocellular carcinoma cells in vitro and in vivo, and elucidated the relationship between

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service